20 May, 2021
The wMicroTracker One instrument can be used to measure the locomotor activity of parasitic nematodes cultured in liquid media and in multi-well plates.
The conventional methods of measuring anthelmintic efficiency on parasitic nematode are laborious and low throughput. The wMicroTracker instrument can be used for library screening and drug discovery in a variety of parasitic nematodes.
The wMicroTracker ONE has been tested and validated for the following parasitic species:
| Species | Stage | Lenght in µm |
| Echinococcus granulosus (5) | protoscolex | 200 |
| Mesocestoides corti (1,4) | tetrathyridia | 750-1000 |
| Haemonchus contortus (3,7) | L3 | 730-805 |
| Cooperia oncophora (3) | xL3 | 865-940 |
| Teladorsagia circumcincta (3) | xL3 | 830-865 |
| Ostertagia ostertagi (3) | xL3 | 850-915 |
| Dirofilaria immitis | larvae | 300 |
| Brugia pahangi (2) | microfilariae/ L3 | 177-230 / 1000-2000 |
| Schitosoma mansoni (2) | schistosomules | 110 |
| Meloidogyne incognita | J2 | 350-450 |
| Angiostrongylus cantonensis (6) | L3 | 425-524 |
| Heligmosomoides polygyrus (8) | egg hatching – L1 – L2 | 300-600 |
- The potential for histone deacetylase (HDAC) inhibitors as cestocidal drugs. Hugo R Vaca, Ana M Celentano, María A Toscanini, Tino Heimburg, Ehab Ghazy, Patrik Zeyen, Alexander-Thomas Hauser, Guilherme Oliveira , María C Elissondo, Manfred Jung, Wolfgang Sippl, Federico Camicia, Mara Cecilia Rosenzvit. PLoS Negl Trop Dis. Mar 2021.
- In vitro screening methods for parasites: the wMicroTracker & the WormAssay. Emma Gunderson, Christina Bulman, Mona Luo, Judy Sakanari. microPublication Biology. July 2020.
- Screening of a drug repurposing library with a nematode motility assay identifies promising anthelmintic hits against Cooperia oncophora and other ruminant parasites. Liu M., Landuyt B., Klaassen H., Geldhof P., Luyten W. Veterinary Parasitology. 2018 Dec.
- Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes. Rosenzvit MC et al. PLoS Negl Trop Dis. Feb 2018.
- The nervous and prenervous roles of serotonin in Echinococcus spp. Camicia F, Herz M, Prada LC, Kamenetzky L, Simonetta SH, Cucher MA, Bianchi JI, Fernández C, Brehm K, Rosenzvit MC. Int J Parasitol. Jul 2013.
- A validated high-throughput method for assaying rat lungworm (Angiostrongylus cantonensis) motility when challenged with potentially anthelmintic natural products from Hawaiian fungi. Randi L. Rollins, Mallique Qader, William L. Gosnell, Cong Wang, Shugeng Cao and Robert H. Cowie. Parasitology. 2022 Mar 3;1-28.
- High-Throughput Phenotypic Assay To Screen For Anthelmintic Activity On Haemonchus Contortus. Aya C. Taki, Joseph J. Byrne, Tao Wang, Brad E. Sleebs, Nghi Nguyen, Ross S. Hall, Pasi K. Korhonen, Bill C.H. Chang, Paul Jackson, Abdul Jabbar, and Robin B. Gasser. Pharmaceuticals 2021, 14, 616.
- Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated HighThroughput Method. Yamssi Cédric , Noumedem A C Nadia, Simeni N Sylvain Raoul, Samuel Berinyuy, Mounvera A Azizi , Tientcheu N J Sandra , Ngouyamsa N A Sidiki , and Vincent K Payne. Journal of Tropical Medicine 2023.