17 April, 2024
Parasitic worm infections are commonly treated with drugs that either paralyze the worms or inhibit their reproduction. With the use of WMicrotracker, it becomes feasible to identify novel small molecules, genes, or natural compounds capable of inhibiting egg hatching. Below, we illustrate an example of its application.
Linearity of Egg Hatching Detection
This experiment aims to observe the kinetics and linearity of C.elegans egg hatching detection. As larvae emerge, they commence movement and are subsequently detected by the system.
Methodology:
- Culture populations of worms on NGM plates.
- Synchronize the worms using the hypochlorite method.
- Quantify the number of eggs in 5 μl and adjust the volume to achieve the desired egg concentration [5-20 eggs/10 μl].
- Transfer 100 μl of the egg solution to 96-well microplates using a multichannel pipette. Optionally, at this stage, chemical compounds can be added to assess their potential toxic properties.
- Register the plate with WMicrotracker